Testicular GCTs are mutationally quiet other than frequent gain at 12p but had not yet been comprehensively examined for gene fusions. Next generation RNA sequencing is now the best available technology for detecting fusions not visible through standard techniques such as FISH or RT-PCR. The group examined embryonal carcinoma cell lines as well as 24 testicular GCTs with different histologic subtypes. They found 8 previously undescribed fusion transcripts commonly involving the RCC1 gene or internal translocations of ETV6.
* This study reveals that testicular GCTs are more marked by fusions than by mutations similar to childhood sarcomas.