Changing practice: red blood cell typing by molecular methods for patients with sickle cell disease

Changing practice: red blood cell typing by molecular methods for patients with sickle cell disease

Casas et al, 2015 Transfusion

Link to abstract: http://onlinelibrary.wiley.com/doi/10.1111/trf.12987/abstract

 

Retrospective study of RBC antigen phenotypes and genotypes, as per pre-transfusion samples done routinely at 1 year of age. Compared RBC antigen phenotypes obtained by hemagglutination methods and by genotyping predictions.

Genotyping was done using DNAbased assays targeting SNP associated with blood group Ag expression for 35 antigens. 494 patients (single-institution (CHOP), 2008-2014), total 6360 antigen comparisons; Results: 77 discrepancies (1.1%): 16 Fyb, 13 Jkb, 10 M, 10 N, 7 S. Serologic assays were redone for 66; 64 in concordance with genotyping –> concordance 0.9997. 34 false-positive serologic testing; 33 false-negative serologic testing and 15 false negative serologic results associated with alleles encoding weak antigens or single-dose Fyb expression.